科研人必看：多肽、蛋白质、重组蛋白搞不清？ 应用场景有哪些？定制如何选？_MedChemExpress (MCE)

{"type":"doc","content":[{"type":"paragraph","attrs":{"id":"ab851e38-2163-4a82-98ab-c0ef073a32ee","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"Section.01"}]},{"type":"paragraph","attrs":{"id":"ec222d85-c3ef-4b0f-9b73-8db41682df58","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"多肽 VS 蛋白质 VS 重组蛋白"}]},{"type":"paragraph","attrs":{"id":"f24be777-710e-49ec-8100-dd3c9de285c6","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"60796891-f3ee-45b8-a49e-3f0767cbea13","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"多肽、蛋白质和重组蛋白本质上都是由氨基酸组成的生物大分子，三者的主要区别在于分子大小、折叠结构以及生产方式："}]},{"type":"paragraph","attrs":{"id":"dc8640ca-69b4-4b4e-9072-a4cb6d26293a","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"image","attrs":{"id":"d2725228-7fb1-4abe-80bc-aeaf0b26a092","src":"https://developer.qcloudimg.com/http-save/audit-10281355/7fa44aaf119f29f06eef0c730b4362e6.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"image","attrs":{"id":"baccb8a6-989b-4e7c-b8aa-531b77df6c1b","src":"https://developer.qcloudimg.com/http-save/audit-10281355/820ec867cb1dbc8ec24a00f5d5dad54f.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"72670183-e854-4930-a357-8f691acd5b39","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":" "}]},{"type":"paragraph","attrs":{"id":"4a9b390b-d23b-4997-8eb6-1c978eef9872","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"弄清区别只是第一步。在实际应用中，抗体筛选、靶点验证、药物递送等场景，经常需要"},{"type":"text","marks":[{"type":"bold"}],"text":"特定序列的多肽"},{"type":"text","text":"或"},{"type":"text","marks":[{"type":"bold"}],"text":"非天然来源的重组蛋白"},{"type":"text","text":"。这时候，成品库里的选项远远不够。于是，您需要选择"},{"type":"text","marks":[{"type":"bold"}],"text":"「多肽 & 重组蛋白定制服务」"},{"type":"text","text":"。"}]},{"type":"paragraph","attrs":{"id":"db56cd36-428b-49e2-a346-3a3126b2772b","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"Section.02"}]},{"type":"paragraph","attrs":{"id":"8208b955-7af2-43c0-b716-0d8afc857d80","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"多肽定制"}]},{"type":"paragraph","attrs":{"id":"d13568b5-0b31-4903-88a3-fb1a37b1e9e5","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"4e5018c3-9697-436b-bc65-264fb4458790","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"一、什么是多肽定制？"}]},{"type":"paragraph","attrs":{"id":"17316c91-5c5d-4845-a3aa-0127c70cdc3c","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"多肽定制！"},{"type":"text","text":"是的，在科研研究中有时要根据具体需求，如特定的氨基酸序列、分子量、纯度和修饰方式等，利用化学或生物技术人工合成特定的多肽，也就是多肽定制。"}]},{"type":"paragraph","attrs":{"id":"b548d825-b476-485d-92a4-abc858f3481e","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"a3fbb29a-d226-47d5-86a5-a29297d67d3c","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"二、为什么要定制多肽？"}]},{"type":"paragraph","attrs":{"id":"f67ad44f-0da8-46f8-83cf-96fb09414a70","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"当然是因为：有需求！"}]},{"type":"paragraph","attrs":{"id":"27c82fff-671d-4841-b181-cdca70e1e4d0","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"比如："}]},{"type":"paragraph","attrs":{"id":"43fb2b22-a8ad-4d6d-b666-7cd58c0cf5e1","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"(1)高度个性化"},{"type":"text","text":"：目录产品多肽无法满足实验需求，定制可以贴合实验需求进行设计，甚至加入同位素标记、荧光标记"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如 FITC、Cy3)"},{"type":"text","text":"等特殊基团以追踪细胞内反应。"}]},{"type":"paragraph","attrs":{"id":"ae9e9efc-2117-42c1-ac86-6e786e5b8cce","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"(2)提高稳定性与活性"},{"type":"text","text":"：可通过特殊的化学修饰"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如 N 端乙酰化、C 端酰胺化、环化)"},{"type":"text","text":"来抵抗生物体内酶的降解，增强药效或生物利用度。"}]},{"type":"paragraph","attrs":{"id":"d7788fe7-b543-4de6-b357-e27268ffa029","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"(3)结构与功能研究"},{"type":"text","text":"：用于抗体制备、受体研究、多肽文库筛选以及酶活性位点的探索。"}]},{"type":"paragraph","attrs":{"id":"c929d4ad-9e86-43a7-9bcb-66648ea95670","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"(4)新药与功能性产品开发"},{"type":"text","text":"：用于开发多肽相关药物等。"}]},{"type":"image","attrs":{"id":"62bffb6f-b27e-46f7-ac11-b05444988c05","src":"https://developer.qcloudimg.com/http-save/audit-10281355/d931bb8ba427d99eb5c1acf241d5eaf3.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"1f7d433b-3a55-4395-a282-c2e02db83d90","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 1. 多肽定制的常见类型。"}]},{"type":"paragraph","attrs":{"id":"1dac259a-eff4-484c-a6fe-bf66b839503c","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"接下来我们梳理几种常见的多肽定制场景，结合文献应用帮助大家更好地选择~"}]},{"type":"paragraph","attrs":{"id":"3715030c-04e8-45ca-b3de-cfb663142fdb","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"8ab0cd5b-1175-48f0-8505-d5d27fc179e5","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景一： 荧光标记肽"}]},{"type":"paragraph","attrs":{"id":"bf54d6b8-8c5b-480a-9f9d-3a896b38312d","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"荧光标记肽是"},{"type":"text","marks":[{"type":"bold"}],"text":"应用非常广泛的功能化多肽"},{"type":"text","text":"，通过在多肽的 N 端、C 端或特定位点"},{"type":"text","marks":[{"type":"bold"}],"text":"偶联 FAM、FITC、Cy5、TAMRA 等荧光染料，"},{"type":"text","text":"实现对多肽或多肽识别分子在细胞、组织、活体中的"},{"type":"text","marks":[{"type":"bold"}],"text":"动态追踪。"},{"type":"text","text":"相比传统放射性标记，荧光标记具有操作更安全、成像更直观、适用于活细胞实验等优势，被广泛用于受体结合、细胞摄取等研究领域。"}]},{"type":"paragraph","attrs":{"id":"6ccdc6cf-d593-46e3-a3d6-e8d77272cfa8","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"需要注意的是，荧光团类型与标记位点会直接影响多肽的受体结合能力与生物学功能，甚至导致活性显著下降或完全丧失，如下这篇文献探究了不同荧光团标记"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"Substance P"},{"type":"text","text":"后多肽与受体结合的活性差异。在进行荧光标记肽的设计时，大家也应该参考文献报道和活性区域进行选择。"}]},{"type":"image","attrs":{"id":"269b63c0-22db-44a2-831f-e1804518637d","src":"https://developer.qcloudimg.com/http-save/audit-10281355/140394db838c1c74af8f25c5c8706502.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"8b8a7007-8642-4249-888a-a183ee03f96e","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 2. 不同荧光团标记 Substance P 在 CHO 细胞中的成像图[1]。"}]},{"type":"paragraph","attrs":{"id":"6c6710b9-f506-4dfe-968e-ed28411058f0","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(A) Alexa 488-SP, (B) BODIPY Fl-SP, (C) fluo-rescein-SP, (D) Oregon Green 488-SP, (E) tetramethylrhodamine-SP, (F) PBS"}]},{"type":"paragraph","attrs":{"id":"b018ad1a-928e-4347-aa13-1261ce299528","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"除了单荧光标记肽，基于荧光团/淬灭基团标记"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"FRET 多肽"},{"type":"text","text":"则通过巧妙的设计，将分子水平的酶活响应和结构变化结构变化转化为荧光信号的输出，提供了灵敏、快速的检测工具。"}]},{"type":"paragraph","attrs":{"id":"c570ef1e-55cc-4df6-a359-ea1b7e469f64","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景二：脂肪酸修饰肽"}]},{"type":"paragraph","attrs":{"id":"de821639-4afa-456a-b6f8-3eeb8db4bb95","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"脂肪酸修饰是目前"},{"type":"text","marks":[{"type":"bold"}],"text":"应用最成熟的长效化多肽修饰策略"},{"type":"text","text":"，通过"},{"type":"text","marks":[{"type":"bold"}],"text":"引入棕榈酸、硬脂酸等疏水链"},{"type":"text","text":"，使多肽能够与血清白蛋白发生可逆结合，从而显著"},{"type":"text","marks":[{"type":"bold"}],"text":"降低肾清除率并延长体内停留时间"},{"type":"text","text":"。"}]},{"type":"paragraph","attrs":{"id":"314855ef-b3a5-4541-a8da-81a4d67f0a32","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"在 GLP-1 类似物中，脂肪酸修饰肽已经多次优化并获得了临床的成功应用。"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"Liraglutide"},{"type":"text","text":"通过在 Lys26 位连接棕榈酸"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(C16)"},{"type":"text","text":"，使其与白蛋白高亲和结合，从而将天然 GLP-1 约 2 分钟的半衰期延长至约 13 小时，实现每日给药。在此基础上进一步优化的"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"Semaglutide"},{"type":"text","text":"则采用 C18 脂肪二酸结合改造，使其半衰期延长至约一周，支持每周一次给药方案。"}]},{"type":"image","attrs":{"id":"529fc43d-f124-4b88-916f-a5eb718f10f4","src":"https://developer.qcloudimg.com/http-save/audit-10281355/2834200277841e28826cd51277c72adb.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"52f977ca-3c9d-44ff-aad4-ae1bf82ec5ad","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 3. 脂肪酸修饰的生物学价值：延长半衰期、降低免疫原性，细胞内摄取及跨上皮屏障递送[2]。"}]},{"type":"paragraph","attrs":{"id":"8a8ac32a-e711-4a71-b994-c0e78dc80544","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"2d3434b7-d37b-4cc5-b203-7d3f1bd2007c","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景三：同位素标记肽"}]},{"type":"paragraph","attrs":{"id":"e53148ec-d5ab-4e1d-9f37-cd888770e371","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"同位素标记肽是"},{"type":"text","marks":[{"type":"bold"}],"text":"目前蛋白质组学与定量分析中最标准化的一类多肽定制形式"},{"type":"text","text":"，其核心是通过"},{"type":"text","marks":[{"type":"bold"}],"text":"引入"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"13"},{"type":"text","marks":[{"type":"bold"}],"text":"C、"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"15"},{"type":"text","marks":[{"type":"bold"}],"text":"N 或"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"2"},{"type":"text","marks":[{"type":"bold"}],"text":"H 等稳定同位素"},{"type":"text","text":"，"},{"type":"text","marks":[{"type":"bold"}],"text":"在质量数上构建“重/轻肽对”"},{"type":"text","text":"，从而在 LC-MS/MS 体系中"},{"type":"text","marks":[{"type":"bold"}],"text":"实现高精度绝对定量或相对定量分析"},{"type":"text","text":"。典型策略包括 AQUA"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Absolute Quantification)"},{"type":"text","text":"肽，即在已知浓度基础上合成稳定同位素标记肽作为内标，用于目标蛋白或修饰肽的精确定量分析。"}]},{"type":"paragraph","attrs":{"id":"0d5103b6-6c4a-4d5f-bd39-24abbd32bc3d","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"在实际应用中，同位素标记肽被广泛用于靶向蛋白定量"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(SRM/MRM/PRM)"},{"type":"text","text":"、生物标志物验证以及信号通路定量分析。例如，在膜蛋白与 GPCR 研究中，通过向样品中加入已知浓度的重标记肽标准，可以直接计算内源肽的绝对含量，从而避免传统抗体法带来的批次差异与交叉反应问题。同时，由于重标记与天然肽在化学性质上几乎一致"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(仅质量差异)"},{"type":"text","text":"，两者在色谱行为和离子化效率上具有高度一致性，使其成为定量体系中最可靠的内部参照物之一。"}]},{"type":"paragraph","attrs":{"id":"29eca95c-fce9-4109-b910-390a032b9d94","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"d65d7e8c-6eb7-46c5-bb71-386ebbde4ebf","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景四：点击化学修饰肽"}]},{"type":"paragraph","attrs":{"id":"436b3603-3cbc-4eb9-b7db-5e6394323f5b","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"点击化学修饰肽是近年来发展非常迅速的一类功能化多肽定制策略，其核心是利用叠氮"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(–N3)"},{"type":"text","text":"与炔基"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(–C≡C)"},{"type":"text","text":"等生物正交反应基团，在温和条件下实现高选择性偶联，从而将荧光团、小分子药物或纳米材料精准与多肽偶联。点击化学本质上属于“模块化连接策略”，其优势不仅在于反应效率高，更重要的是可以实现不同功能模块"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(靶向肽、成像基团、药物载荷)"},{"type":"text","text":"之间的快速组合，因此点击化学修饰已成为 PDC、放射性核素偶联药物以及多功能多肽构建中最常用的偶联方式之一。"}]},{"type":"image","attrs":{"id":"551c4009-79ae-422e-8e27-c3bd27515be3","src":"https://developer.qcloudimg.com/http-save/audit-10281355/90a086e53fab27b47b48f0e7f8195f47.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"af170c9f-49ba-4a68-8c74-588a8aca6a7e","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 4. 通过点击化学连接寡糖和多肽链的化学结构示例[3]。"}]},{"type":"paragraph","attrs":{"id":"befcbd2d-5f7b-4ffe-a3aa-48430139c0d8","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"b28a125e-286b-44b3-9be1-a8cd519f4693","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景五：DSPE-PEG-多肽偶联物"}]},{"type":"paragraph","attrs":{"id":"82965747-735a-47b5-992c-c72912dd2ad1","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"DSPE-PEG-多肽偶联物是目前脂质体与纳米递送体系中最常用的表面功能化策略之一，其基本结构由疏水性 DSPE 脂质锚定在膜结构中，亲水性 PEG 作为柔性间隔臂，末端连接靶向多肽，从而在保证体系稳定性的同时赋予其特异性识别能力。这类结构被广泛用于靶向脂质体、纳米颗粒及核酸递送体系构建中，用于提升体内循环稳定性并实现组织或细胞特异性摄取。"}]},{"type":"paragraph","attrs":{"id":"488b634b-f572-4e61-a6fe-5cc267d1619a","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"在纳米递送体系中，DSPE-PEG-多肽偶联物常与细胞穿膜肽"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Cell-Penetrating Peptide, CPP)"},{"type":"text","text":"协同使用，以同时实现体内稳定性与细胞内高效递送，例如在其表面引入 TAT 肽，构建出 DSPE-PEG–TAT 共修饰纳米载体，用于实现肿瘤或组织中的高效细胞摄取与递送。"}]},{"type":"image","attrs":{"id":"3cc201ef-2c3d-4b76-bf7d-0dbb9f50c8d2","src":"https://developer.qcloudimg.com/http-save/audit-10281355/a55c1e347cb7af2cafa63c3fea79a919.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"d46ecf51-239b-4835-9dda-402f6b4b13d3","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 5. 转铁蛋白与细胞穿膜肽双功能修饰肽用于体内外递送质粒以治疗阿尔茨海默病[4]。"}]},{"type":"paragraph","attrs":{"id":"8d32020f-0f32-4850-99bf-ba4bf39c3e69","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"ace8de74-cf9b-495a-b6bc-ab1088020a38","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"场景六：多肽-药物偶联物(PDC)"}]},{"type":"paragraph","attrs":{"id":"e9335147-15e0-44ce-8e33-72cfe32bad77","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"多肽-药物偶联物"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Peptide-drug conjugate, PDC)"},{"type":"text","text":"指的是利用具有靶向能力的多肽，将小分子药物、毒素、放射性核素或功能分子定向递送至目标组织。相比传统化疗药物，PDC 通常具有更好的靶向性和相对较低的毒副作用；而相比抗体-药物偶联物"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Antibody-drug conjugate, ADC)"},{"type":"text","text":"，PDC 具有分子量小、化学合成灵活易变的优势。"}]},{"type":"image","attrs":{"id":"9ac134ac-85e9-4c66-822c-b923952a2dea","src":"https://developer.qcloudimg.com/http-save/audit-10281355/27ee6bc5ac3a4a70ec80e3616c6e3eb2.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"4f5590a8-1b51-450a-9d74-9a72fce44e4a","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 6. PDC 药物结构示意图[5]。"}]},{"type":"paragraph","attrs":{"id":"f30d01f2-38a9-4466-825f-8d2d608c95ab","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"已获批上市的 Lutathera"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(177Lu-"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"DOTATATE"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":")"},{"type":"text","text":"通过将生长抑素类似物与 Lu-177 偶联，实现神经内分泌肿瘤的靶向放疗；而"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"ANG1005"},{"type":"text","text":"则由三个紫杉醇分子与"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"underline"}],"text":"Angiopep-2"},{"type":"text","text":"肽段共价连接组成，通过低密度脂蛋白受体相关蛋白运输系统穿越血脑屏障，用于脑肿瘤的研究与治疗。"}]},{"type":"paragraph","attrs":{"id":"b5695716-c102-4fd2-b4b3-798a4b6bd1b9","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"Section.03"}]},{"type":"paragraph","attrs":{"id":"91413904-9bc9-4257-a28b-ee6b0c1bf0a3","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}}],"text":"重组蛋白定制"}]},{"type":"paragraph","attrs":{"id":"091d5bf5-7d78-4fb8-9a39-cee1d7c24238","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"f3e92ebb-5877-47aa-bf0e-de46a20b2b10","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"蛋白质可以分为传统天然蛋白质和重组蛋白两大类。"}]},{"type":"paragraph","attrs":{"id":"f8dfe4eb-a462-44a9-8c82-313a05e65e10","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"传统天然蛋白质是直接从动植物组织或微生物中提取获得"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如大豆蛋白、乳清蛋白、胶原蛋白)"},{"type":"text","text":"，属于自然界中原本就存在的形式，保留了完整的天然结构与复杂的翻译后修饰"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如糖基化)"},{"type":"text","text":"。"}]},{"type":"paragraph","attrs":{"id":"babf3882-e1ea-4c7e-9efd-9eb942648b9b","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"重组蛋白"},{"type":"text","text":"是利用基因工程技术，将目的基因导入宿主细胞"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如大肠杆菌、酵母菌或哺乳动物细胞)"},{"type":"text","text":"中进行发酵表达而生产的蛋白质。不仅能生产天然存在的蛋白质，还可以通过改造氨基酸序列来增强特定功能。具有纯度高、批次稳定、安全性好及易于规模化制备的优势。"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"主要用于生产重组人胰岛素、细胞因子、单克隆抗体等靶向药物。1982 年重组人胰岛素"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Humulin，优泌林)"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"被 FDA 批准为首个重组蛋白药物。"}]},{"type":"image","attrs":{"id":"065fb181-3f9a-4587-8b3f-59ced892cbdf","src":"https://developer.qcloudimg.com/http-save/audit-10281355/25591d807acf2d114e40adba72e26cc5.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"a922de73-815c-4fb1-bf17-611c1027b3c1","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 7. MCE 重组蛋白定制基本流程。"}]},{"type":"paragraph","attrs":{"id":"61e7c490-0670-4ef0-94e2-f5c0ac02bd26","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"71c090b1-2a47-45ed-89d7-04b92e52221b","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"常见重组蛋白类型"}]},{"type":"paragraph","attrs":{"id":"90af270f-7172-487d-a5df-d64fc8c22d3f","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"『"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"bold"}],"text":"五大表达系统"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"』"}]},{"type":"paragraph","attrs":{"id":"9cc00ed5-ae51-4cf7-86d4-abc8e68ab20e","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"不同表达系统各有特点，可以根据蛋白特性和后续应用灵活匹配。"}]},{"type":"paragraph","attrs":{"id":"ba196cb6-7461-4d04-b3e9-fcc599973137","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"大肠杆菌表达："},{"type":"text","text":"经济、高产、快速，适合结构相对简单的细菌蛋白、抗原、酶类等，是性价比最高的选择。"}]},{"type":"paragraph","attrs":{"id":"a90597e7-d722-4286-8cdc-4fd0d3fc679f","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"大肠杆菌无细胞表达："},{"type":"text","text":"体外合成，无需活细胞培养，特别适合对宿主有毒性的蛋白以及多次跨膜蛋白，可添加去垢剂维持膜蛋白溶解状态。"}]},{"type":"paragraph","attrs":{"id":"b5a346ce-ae55-4991-9272-6ea1c86fcccf","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"酵母细胞表达："},{"type":"text","text":"低成本的真核表达平台，具备一定的翻译后修饰能力，适合分泌蛋白及小分子蛋白。"}]},{"type":"paragraph","attrs":{"id":"a495cbe4-49af-4fec-ba60-63e3b5c9d918","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"哺乳动物细胞表达："},{"type":"text","text":"最接近天然状态的表达平台，提供完整的人源化翻译后修饰"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(如糖基化、磷酸化)"},{"type":"text","text":"，适合重组抗体、细胞因子等需要复杂修饰的蛋白。"}]},{"type":"paragraph","attrs":{"id":"b8b20f58-846b-47ed-98fb-2f91ac72e79c","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"昆虫细胞表达："},{"type":"text","text":"基因承载容量大，支持大分子量蛋白和激酶的表达。"}]},{"type":"paragraph","attrs":{"id":"6cd3bf7c-e8e3-4092-9ef2-28eedba77eae","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"『"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"bold"}],"text":"多种融合标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"』"}]},{"type":"paragraph","attrs":{"id":"14aff9fb-33fd-4707-908a-8986da63b86a","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"标签的选择直接影响纯化效率和下游应用，MCE 支持多种融合标签，可根据实验需求灵活搭配。"}]},{"type":"paragraph","attrs":{"id":"17ef2816-8de0-4798-a953-790047f0d8a5","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"常规纯化标签："},{"type":"text","text":"His 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(镍柱纯化，分子量小)"},{"type":"text","text":"、GST 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Glutathione 亲和纯化，兼具促溶作用)"},{"type":"text","text":"、Fc 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Protein A/G 亲和纯化，适合免疫相关实验)"},{"type":"text","text":"、Flag 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Anti-FLAG 亲和凝胶纯化，温和洗脱，保持活性)"},{"type":"text","text":"、Strep 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Strep-Tactin 亲和纯化，高纯度需求优选)"}]},{"type":"paragraph","attrs":{"id":"430cb5ac-e106-4bc7-963c-2b0b75439597","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"促溶标签："},{"type":"text","text":"MBP、SUMO、GST 等，可显著提高难表达蛋白的可溶性"}]},{"type":"paragraph","attrs":{"id":"b7318fe3-f1de-4571-9a6c-4ef7ee2726dd","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"功能标签："},{"type":"text","text":"Avi 标签"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(常用于定点生物素化)"},{"type":"text","text":"、GFP"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(荧光标记，用于蛋白定位示踪)"}]},{"type":"paragraph","attrs":{"id":"8f3faa0d-dec9-45aa-8f51-d5a72f376da3","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"无标签蛋白："},{"type":"text","text":"如需天然构象或去除标签干扰，MCE 也支持无标签蛋白制备"}]},{"type":"paragraph","attrs":{"id":"aad36958-5596-468f-bf2d-b8110942d4d6","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"『"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"bold"}],"text":"定制需求"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"』"}]},{"type":"paragraph","attrs":{"id":"fbe05162-5716-4686-9881-85675ac70b39","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"膜蛋白："},{"type":"text","text":"拥有三大膜蛋白表达技术平台，包括去垢剂平台"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(基于哺乳动物细胞表达系统)"},{"type":"text","text":"、纳米盘"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(Nanodisc)"},{"type":"text","text":"技术平台和病毒样颗粒"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"(VLPs)"},{"type":"text","text":"技术平台，通过为膜蛋白提供模拟天然脂质环境，有效维持其天然构象与生物活性。"}]},{"type":"paragraph","attrs":{"id":"cf7488ba-4364-45a3-bd24-490ca7003740","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"生物素化标记："},{"type":"text","text":"通过随机标记或者 Avi 定点生物素化，适用于蛋白相互作用研究"}]},{"type":"paragraph","attrs":{"id":"16ada08e-b149-47aa-a753-870e3a20c19b","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"荧光标记："},{"type":"text","text":"融合 GFP、mCherry 等荧光蛋白，或化学标记 FITC、Cys 系列染料，用于细胞成像及示踪实验"}]},{"type":"paragraph","attrs":{"id":"fcd6e189-ae23-4038-a351-8cf0921485bb","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"包涵体复性："},{"type":"text","text":"对于原核系统中形成包涵体的蛋白，可提供变复性服务，筛选最优复性条件，尽可能恢复蛋白活性"}]},{"type":"paragraph","attrs":{"id":"2c0d54ef-8001-4450-a6be-073d40409d0a","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"突变体及截短体："},{"type":"text","text":"点突变、结构域删除、截短体等，均可按需构建并表达纯化"}]},{"type":"paragraph","attrs":{"id":"b64cfe31-6763-4441-98b6-12ce7a7b6f06","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":"5259242b-1143-4a45-9d86-5c14bf40ca88","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"","background":""}},{"type":"bold"}],"text":"案例分享"}]},{"type":"paragraph","attrs":{"id":"b45567eb-a5eb-479d-816d-8aaf4bdd269a","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"『"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"bold"}],"text":"无细胞表达"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"』"}]},{"type":"paragraph","attrs":{"id":"142bf2f9-a755-4228-815c-2a86466eedca","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"▶解决高疏水跨膜蛋白难题"}]},{"type":"paragraph","attrs":{"id":"bf4487c0-c4e8-40d5-ac47-8742920587d1","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"难点："},{"type":"text","text":"人源 SLC7A11 蛋白，含 12 次跨膜结构，表达量低、可溶性差，纯化困难"}]},{"type":"paragraph","attrs":{"id":"00b85a3a-898e-45ef-aa6e-473c0a522a1c","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"制备："},{"type":"text","text":"采用大肠杆菌无细胞体系，通过添加 DNA 模板、细胞抽提物及其他原料实现蛋白的体外合成；利用 Brij78、FOS12、DDM 等去垢剂，能够有效促进膜蛋白溶解，维持其天然构象。"}]},{"type":"image","attrs":{"id":"ce478e73-fe9e-4990-aa9a-0ed39c786a77","src":"https://developer.qcloudimg.com/http-save/audit-10281355/1caf97b1eb8cac1c881d9bfe9e234399.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"96198731-8fde-4fca-84d7-b36c6b75dc5b","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 8. 纯化结果图和活性验证图。"}]},{"type":"paragraph","attrs":{"id":"c22f166a-fa20-43a6-a9bb-3c0b15b21e58","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"『"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}},{"type":"bold"}],"text":"昆虫细胞表达"},{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(122, 79, 214)","background":""}}],"text":"』"}]},{"type":"paragraph","attrs":{"id":"668f50b5-77aa-443a-995a-3696c7103477","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"▶攻克超大分子量蛋白制备"}]},{"type":"paragraph","attrs":{"id":"c0c449f1-d266-4932-a559-91216d507199","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"项目难点："},{"type":"text","text":"人源 HLTF 蛋白，分子量高达 115 kDa，大分子蛋白分泌困难、表达量低，纯化难度大"}]},{"type":"paragraph","attrs":{"id":"5c4c2cb2-7860-49d6-94b0-e1a89002cdcc","textAlign":"justify","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"bold"}],"text":"制备："},{"type":"text","text":"采用杆状病毒介导昆虫细胞表达，迭代优化 P0-P2 代病毒，通过结合亲和、离子交换、疏水及分子筛等多种层析方法纯化目的蛋白。"}]},{"type":"image","attrs":{"id":"1a2c0aa7-5993-40c4-9623-78e676c2d7ce","src":"https://developer.qcloudimg.com/http-save/audit-10281355/bd664a34ca876c032d3e0bd9324df5f9.png","extension":"","align":"center","alt":"","showAlt":false,"href":"","boxShadow":"","width":"","aspectRatio":0,"status":"success","showText":true,"isPercentage":false,"percentage":0,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"2b69243d-f969-4a37-b9a0-3a7783307161","textAlign":"center","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"图 9. 纯化结果图。"}]},{"type":"heading","attrs":{"id":"ee9297c4-1d0b-4311-9b79-965aeb95e2ee","textAlign":"inherit","indent":0,"level":1,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"66a541b1-9a20-4e6f-a0c3-b3b83d3b9172","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":"参考文献"}]},{"type":"paragraph","attrs":{"id":"5d920088-3d1f-4e74-bdc7-46c7948f59cb","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"[1] Simmons MA, et al. Analysis of fluorescently labeled substance P analogs: binding, imaging and receptor activation. BMC Chem Biol. 2001;1(1):1."}]},{"type":"paragraph","attrs":{"id":"7a739aad-fd6d-43c4-8898-c77deafb5c24","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"[2] Lynch MD, et al. A review of lipidation in the development of advanced protein and peptide therapeutics. J Control Release. 2019 Feb 10;295:1-12."}]},{"type":"paragraph","attrs":{"id":"c3d4044d-7bc0-4ee7-8975-5da04f67c617","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"[3] Danishefsky SJ, et al. A potentially valuable advance in the synthesis of carbohydrate-based anticancer vaccines through extended cycloaddition chemistry. J Org Chem. 2006 Oct 13;71(21):8244-9."}]},{"type":"paragraph","attrs":{"id":"e2ca172d-a115-4e61-ac46-b315b7ac4812","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"[4] Singh J, et al. Synthesis and Characterization of Transferrin and Cell-Penetrating Peptide-Functionalized Liposomal Nanoparticles to Deliver Plasmid ApoE2 In Vitro and In Vivo in Mice. Mol Pharm. 2025 Jan 6;22(1):229-241."}]},{"type":"paragraph","attrs":{"id":"f251e382-6c14-4dca-b3ac-d47ba59c3c03","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","marks":[{"type":"textStyle","attrs":{"color":"rgb(136, 136, 136)","background":""}}],"text":"[5] Bugatti K. A Brief Guide to Preparing a Peptide-Drug Conjugate. Chembiochem. 2023 Sep 1;24(17):e202300254."}]},{"type":"paragraph","attrs":{"id":"f8fb4746-e9b1-4f6d-aba5-17223e92352f","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false}},{"type":"paragraph","attrs":{"id":"c9b23e6f-3025-4cbc-ad08-94ee41e7fbca","textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false},"content":[{"type":"text","text":""}]},{"type":"paragraph","attrs":{"id":null,"textAlign":"inherit","indent":0,"color":null,"background":null,"isHoverDragHandle":false}}]}","createTime":1783392656,"ext":{"closeTextLink":0,"comment_ban":0,"description":"","focusRead":0},"favNum":0,"html":"","isOriginal":0,"likeNum":0,